Table 1.
Differentially expressions of miRNAs 231/E1A cells versus 231/VC cells were analyzed by miRNA microarray analysis and confirmed by qRT-PCR.
| Upregulated miRNAs by E1A | ||||||
|---|---|---|---|---|---|---|
| miRNA ID | microarray | qRT-PCR# | P-value | Function | Validated target | Refs. |
| has-mir-377 | 78.6 | 3.48±1.39 | 0.0199* | unknown | unknown | |
| has-mir-515-3p | 12.67 | 13.87±7.66 | 0.0102* | unknown | unknown | |
| has-mir-92 | 7.7 | 3.13±0.41 | 0.0450* | unknown | unknown | |
| has-mir-18a | 6.27 | 6.93±1.05 | 0.0121* | inhibition cell proliferation | K-Ras | (53) |
| has-mir-15b | 6.24 | 5.11±1.04 | 0.0051** | cell proliferation, apoptosis, cell cycle progression | cyclin E1 | (54), (55) |
| has-mir-195 | 4.71 | 3.24±1.49 | 0.0583 | block G1/S transition | cyclin D1, CDK6, E2F3 | (56) |
| has-mir-182 | 4.18 | 3.05±0.91 | 0.0548 | transformation or maintenance of an oncogenic state | FOXO1 | (57) |
| has-mir-107 | 4.06 | 4.26±1.5 | 0.1035 | cell cycle arrest | unknown | (58) |
| has-mir-93 | 3.16 | 3.77±0.34 | 0.0806 | unknown | FUS1 | (59) |
| has-mir-25 | 3.11 | 4.74±1.48 | 0.0878 | unknown | Krüppel-like factor 4 (KLF4) | (60) |
| Downregulated miRNAs by E1A | ||||||
| miRNA ID | microarray | qRT-PCR | P-value | |||
| has-mir-520h | 0.01 | 0.02±0.02 | 0.0001** | stem cell differentiation | ABCG2 | (61), (62) |
| has-mir-197 | 0.02 | 0.78±0.03 | 0.0178* | unknown | FUS1 | (63) |
| Let-7b | 0.23 | 0.30±0.17 | 0.0365* | Induces apoptosis and inhibits tumorigenesis | RAS, MYC and HMGA2 | (64) |
| has-mir-27a | 0.31 | 0.33±0.08 | 0.0352* | cancer cell growth, | Prohibitin, FOXO1 | (65) |
#
Each assay was performed in at least three separate experiments. The data were normalized to the level of U47 RNA in each sample.
The asterisk indicates a significant difference between 231/VC and 231/E1A cells.
*
, P < 0.05.
**
P < 0.01.