Skip to main content
. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: J Nutr Biochem. 2010 Jan 25;21(12):1178–1185. doi: 10.1016/j.jnutbio.2009.10.003

Figure 6.

Figure 6

(A) Confluent human aortic endothelial cell (HAEC) were serum-starved using HBSS buffer for 30 min and then stimulated with various concentrations of ABA (0, 0.01, 0.1, 1, 10 µM) or Forskolin (1µM) for 5 minutes. After removing supernatant, cells were lysated with 0.1 M HCL for 20 minutes to measure non-acetylated cAMP. (B) Confluent HAEC were serum-starved using HBSS buffer for 30 minutes and then stimulated with various concentrations of ABA (0, 0.001, 0.01, 0.1, 1, 10µM) for 15 minutes to measure NO. Results (pmol/ml) from four independent experiments are depicted. (C) Endothelial nitric oxide synthase (eNOS) mRNA expression after 24 hr treatment with control vehicle (DMSO) or ABA (10 µM). Data are represented as mean ± standard error. Points with an asterisk are significantly different from the control (P<0.05).