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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Cryobiology. 2009 Oct 8;60(3S):S60–S65. doi: 10.1016/j.cryobiol.2009.09.009

Figure 2. Apoptosis assay in frozen sheep ovarian explants.

Figure 2

(A) Caspase-3 (B) CD-95 showed apoptotic oocytes in brown. There was no apoptosis in other cells. (C) Oocyte loss and “glassy membrane” presence can be seen in frozen samples, but not in the vitrified grafts. A&B, x200; C, X20.