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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Biochim Biophys Acta. 2009 Oct 30;1802(10):918–923. doi: 10.1016/j.bbadis.2009.10.010

Fig. 2.

Fig. 2

Dynamic tracking of autophagy in primary cortical neurons in vitro. A. Discrimination of GFP-LC3+/− mice using fluorescent macroscopy. B. Identification of GFP-LC3-enriched vesicles suggestive of autophagosomes in primary cortical neurons from GFP-LC3+/− mice after 24 hours of nutrient deprivation using fluorescent microscopy. C. Dynamic tracking of mitophagy in primary cortical neurons from GFP-LC3+/− mice using time-lapsed microscopy. Autophagosomes (green) fusing with mitochondria (red; MitoTracker Red, Invitrogen, Carlsbad, CA) are highlighted by arrows. Time stamp is hours:minutes.