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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Cardiovasc Pathol. 2010 Jan 25;19(6):e233–e240. doi: 10.1016/j.carpath.2009.12.002

Figure 2.

Figure 2

Representative immunoblots of CD-31, smoothelin, vimentin and actin protein expression in cultured fibroblasts isolated from sham-operated hearts (lanes 1 and 2), noninfarcted (lanes 3 and 4) and infarct (lanes 5 and 6) regions of hearts subjected to MI, and tissue homogenates of mouse aorta (ao, lane 7), intestine (int, lane 8) and lung (lane 9) demonstrating that our fibroblast cultures (lanes 1–6) are devoid of measurable endothelial and smooth muscle cell contamination. The endothelial cell marker CD-31, or PECAM-1, is expressed in lung tissue; the smooth muscle cell marker smoothelin is present in aorta and intestine. The nonspecific marker, vimentin, is seen in all lanes.