Figure 3.
Top: Representative immunoblots of Cx43, Cx45, N-cadherin (probed with an anti-pan cadherin antibody), and actin expression in fibroblasts isolated from sham-operated hearts and from noninfarcted and infarcted regions of hearts subjected to coronary ligation. Equal protein (30 μg) was loaded in each lane. Cx43, Cx45 and N-cadherin band densities were normalized to actin band densities to control for protein loading. Bottom: Histograms of summarized immunoblot data showing that Cx43 expression was increased significantly in fibroblasts from both noninfarcted and infarct regions compared to those isolated from sham-operated hearts. *, p < 0.05.
