A. Real-time PCR analysis of TNFα mRNA. Results show the expected upregulation during demyelination in placebo-treated mice, which is reduced by approximately half in E2-treated mice. All samples are normalized to the no cuprizone placebo group.
B. Real-time PCR analysis of IGF-1 mRNA. Results show the expected upregulation during demyelination in placebo-treated mice, which is dramatically reduced in E2-treated mice. All samples are normalized to the no cuprizone placebo group.