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. Author manuscript; available in PMC: 2011 Jun 15.
Published in final edited form as: J Immunol. 2010 May 14;184(12):6637–6648. doi: 10.4049/jimmunol.0902784

Figure 5. Increase in cells is not due to cell death.

Figure 5

3 month old mice were injected with VAD-FMK-FITC 1 hour prior to PEC harvest to detect caspase-positive cells. Cells were analyzed by flow cytometry. (A)The percent VAD-FMK+ cells, and (B) the total number of VAD-FMK+ cells were examined. PEC were harvested from 3 month old mice, stained with Annexin V-FITC and PI, and subsequently analyzed by flow cytometry. Data is expressed as (C) percent or (D) total number of Annexin-V+ or PI+ PEC. For each experiment, n ≥ 4. Statistical significance comparing samples from wild-type and mertk−/−mice is *** p<0.0001 by 2-way ANOVA. (**) p<0.001 by Bonnferoni’s post-test. (*) p<0.05 by Bonnferoni’s post-test.