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. Author manuscript; available in PMC: 2011 Jun 15.
Published in final edited form as: J Immunol. 2010 May 14;184(12):6637–6648. doi: 10.4049/jimmunol.0902784

Figure 8. Expression of chemokines, cytokines, and migration receptors in PEC.

Figure 8

PEC were pooled from at least 4 three month old male mice. RNA was harvested and reverse transcribed to cDNA. A) Real-time PCR was performed for soluble molecules, IL-7, BAFF, CXCL9, CXCL13, and CXCL14. B) Real-time PCR was performed for receptors, CXCR5, CXCR3, and IL-7R. C) PEC from wild-type and mertk−/− mice were double-stained for CD3, CD19, or F480 and CXCR3. Representative histograms of CXCR3 expression gated on CD3+ T cells (D) or CD19+ B cells (E). F) PECs were stained and sorted on the basis of CXCR3 expression. Either CXCR3-negative sorted cells or all cells sorted from mertk−/− donors were stained with cell tracker green and adoptively transferred into mertk−/− recipients. G) PEC from wild-type and mertk−/− mice were double-stained for CD3, CD19, or F4/80 and IL-7R. H) Representative histograms of IL-7R expression CD3+ cells. Mean and standard deviation are graphed. n ≥ 3 per genotype per panel. Statistical significance are indicated, *p<0.05, **p<0.005 by the Student’s t test when comparing wild-type vs mertk−/− mice.