Fig. 3.
Feeding of AFOH to A. parasiticus SRRC2043 or S. cerevisiae: Lane 1, A. parasiticus SRRC2043 (accumulates OMST only) extract; Lanes 2 and 6, AFB1 standard; Lane 3, A. parasiticus SRRC2043 incubated with partially purified AFOH (AFOH is converted to AFB1); Lanes 4 and 10, partially purified synthetic AFOH; Lane 5, OMST standard; Lanes 7–9, AFOH fed to S. cerevisiae expressing norA, ordA, and non-transformed control, respectively. The dotted line indicates the TLC origin. Fluorescent materials at the top of the TLC plates are not shown.