Figure 2.

EP-induced changes in the expression of Ki67. Human corneal stromal cells were doubled stained with phalloidin and Ki67 nuclear proliferative antigen. The cells were cultured in medium containing 8 ng/mL of TGF-β1 and 1% FBS, with or without 15 mM EP for 2 days. The cells were then double stained with anti-Ki67 antibodies (right) and phalloidin (left). There were only a few Ki67-positive cells in the EP-treated cultures.