Figure 4.

Effects of miR-146a on production of IL6, IL8, IRAK1, and PAI-1 in HTM cells. Three individual HTM cell lines were transfected with 146aM or ConM. Three days later, cell culture supernatant and total proteins were collected. IL6 and IL8 levels in cultured supernatant were measured by flow cytometry. Production of IRAK1 and PAI-1 from total protein were determined by Western blot and normalized by β-tubulin (A, B, n = 3). The percentage changes in IL6 and IL8 in 146aM transfected cells compared with their individual ConM-transfected cells. Data are expressed as the mean percentage of change ± SD, n = 3. (C) *#P < 0.05 compared with ConM transfected cells; Mann–Whitney U test.