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. 2003 Dec 1;17(23):2870–2874. doi: 10.1101/gad.1117603

Figure 1.

Figure 1.

Characterization of EEE activity in wild-type and Shh-/- embryos. (A) In 8.5-dpc wild-type embryos, Xgal staining that recapitulates Myf5 expression is seen with BAC140APZ but not with BAC140Δ(EB)Z transgenes. Arrowheads point to the two β-galactosidase-positive cells in the embryo carrying the deletion construct. (B) At 8.5 dpc, Xgal staining is seen in all somites of BAC195Z, Shh-/- embryos as well as their nonmutant littermates. Staining seen in the tail bud of the nonmutant embryo is not in the presomitic mesoderm. In 9.5-dpc nonmutant (C) and Shh-/- (D) embryos carrying BAC195Z, Xgal staining is observed in the myotomes and the dorsal and ventral dermomyotomes of all somites where Myf5 is normally expressed. (E) In 9.5-dpc BAC140Δ(EB)Z, Shh-/- embryos staizned for β-galactosidase, transgene expression is detected in the myotomes and ventral dermomyotomes, but early epaxial expression is missing in all somites.