Figure 8.

Effect of hydrostatic pressure and ruthenium red on ERK phosphorylation. Cells were subjected to 15 mm Hg hydrostatic pressure for 2 hours, then lysed and analyzed by Western blot. Some cells were exposed to 10 μM ruthenium red added for the duration of the experiment. Control cells received neither pressure nor ruthenium red. (A) Representative blot. Lane a, control; lane b, ruthenium red; lane c, 15 mm Hg; lane d, ruthenium red + 15 mm Hg. (B) Pooled data on band density obtained from three independent experiments. ***P < 0.001; significant difference from control. ###P<0.001; significant difference from 15 mm Hg pressure.