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pH profile for 5-MTHF uptake in primary Müller cells. Extravesicular pH was varied from 5 to 8, and uptake was measured for 15 minutes at 37°C. Uptake of [3H]-5-MTHF (40 nM) was measured in the absence (A) and presence (B) of 400 μM TPP in primary mouse Müller cells. Results are mean ± SE of three determinations from at least two experiments.
