Table 1.

Sequences of Primers for Folate Transport Proteins

Gene	NCBI Accession No.	Primer Sequence	Predicted Band Size
Mouse primers (used with primary mouse Müller cells)
FRα	NM_008034	Forward: 5′-GAAGACGAATTCCTGCTGT-3′	419
		Reverse: 5′-TGAGCTTGTAGGAGTGACT-3′
PCFT	NM_026740	Forward: 5′-TGCTAGCCCCTCCGTGTTTGC-3′	567
		Reverse: 5′-CCATCCGGAAGGTGCGACTGT-3′
RFC	NM_031196	Forward: 5′-TCTTTCTAAAGCGCCCTAA-3′	626
		Reverse: 5′-GATACAGGTCTTAAGCGCAGTAGC-3′
Rat primers (used with rat-derived rMC-1 cells)
FRα	NM_133527	Forward: 5′-GTGGATGGCCGAATGTGC-3′	638
		Reverse: 5′-AGAACCTCGCCACTTCCTCGTT-3′
PCFT	BC_089868	Forward: 5′-CACACAGTACATTTGGCACCGCAT-3′	646
		Reverse: 5′-TGGGACCACATACAGCTGGACAAT-3′
RFC	NM_017299	Forward: 5′-CCCTCTTCCTAAAGCGTC-3′	724
		Reverse: 5′-CAGATGATGGACAGCGTCAG-3′