Figure 5.

Apical microvilli were elongated in the Clcn2^nmf240 homozygotes. Images of ezrin staining demonstrated that apical microvilli were elongated in (B, D) the P10 Clcn2^nmf240 homozygotes compared with (A, C) the P10 Clcn2^+/+ littermates. (B, arrows) ezrin-positive staining observed on the basal RPE in the mutant retinas. Magnification: (A, C) ×40; (B, D) ×100. NFL, nerve fiber layer; INL, inner nuclear layer; ONL, outer nuclear layer. (E) The length of the labeled band (WT) or the length of individual processes (mutant) was measured at a minimum of 10 areas in four WT and four mutant retinas, to determine the average apical RPE microvilli length. Error bars, SEM. (F) Western blot analysis demonstrated that levels of ezrin were the same in the Clcn2^nmf240 homozygotes and WT mice. β-Actin served as the loading control.