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. 2003 Dec 1;17(23):2902–2921. doi: 10.1101/gad.1144403

Figure 5.

Figure 5.

The MIND complex contains Mtw1p, Nnf1p, Nsl1p, and Dsn1p. (A) Mtw1p-ProA, Dsn1p-ProA, Nsl1p-ProA, and Nnf1p-ProA co-elute on high-resolution size-exclusion chromatography. (B) Model for the predicted organization of Mtw1p, Dsn1p, Nsl1p, and Nnf1p in the M1, M2, and M3subcomplexes based on the molecular weight of the individual proteins: Mtw1p, 33 kD; Dsn1p, 65 kD; Nsl1p, 25 kD; Nnf1p, 24 kD; ProA tag, 15 kD. (C) Anti-ProA immunoblots of nnf1-17 cells carrying Nsl1p-ProA, Dsn1p-ProA, or Mtw1p-ProA grown at 24°C or 37°C. (D) Crosslinking of Nsl1p-ProA and Dsn1p-ProA to CEN DNA in nnf1-17 cells grown at 24°C or 37°C. (E) Anti-ProA immunoblots of whole-cell extracts, and (F) crosslinking of Nsl1p-ProA, Dsn1p-ProA, and Nnf1p-ProA to CEN DNA in mtw1-1 cells grown at 24°C or 37°C. (G) As a control, extracts were prepared from Mtw1p-ProA okp1-5 cells grown at 24°C or 37°C and analyzed by glycerol-density gradient ultracentrifugation.