Table 2.
Summary of kinetochore subcomplex interdependencies
| Protein | Genetic background | ChIP | Imaging | Western blot | Glycerol-gradient ultracentrifugation |
|---|---|---|---|---|---|
| Ame1p | wild-type | +++ | +++ | +++ | C1 and C2 |
| COMA complexes | |||||
| ndc10-1 | — | N.D.1 | + | N.D. | |
| ndc80-1 | +++ | ++ | + | N.D. | |
| mtw1-1 | +++ | ++ | + | C1 and C2 | |
| COMA complexes | |||||
| okp1-5 | — | — | — | — | |
| Mtw1p2 | wild-type | +++ | +++ | +++ | M1, M2, and M3 |
| ndc80-1 | +++ | ++ | N.D. | N.D. | |
| okp1-5 | +++ | + | ++/+++ | M1, M2, and M3 | |
| MIND complexes | |||||
| nnf1-17 | — | N.D. | — | — | |
| spc25-7 | +++ | N.D. | ++ | N.D. | |
| Ndc80p | wild-type | +++ | +++ | +++ | Ndc80 complex |
| mtw1-1 | +++ | ++ | ++ | N.D. | |
| okp1-5 | +++ | ++ | N.D. | N.D. |
1
N.D.: not determined.
2
Mtw1p-Myc protein levels are unaffected in ndc10-2 cells grown at 37°C (Goshima and Yanagida 2000).