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. 2003 Dec 1;17(23):2933–2949. doi: 10.1101/gad.1145603

Figure 3.

Figure 3.

Histological analysis of Ecsit mutant embryos. (A-E) Wild-type (Ecsit+/+) or heterozygous (Ecsit+/-) embryos (A,C) and homozygous null (Ecsit-/-) embryos (B,D,E) were embedded, sectioned, and stained with hematoxylin and eosin. Adjacent sections were hybridized with the Ecsit2-specific probe to genotype the embryos. Asterisk (*) in D marks delaminating epiblast. In all panels, anterior faces to the left when anterior-posterior orientation can be identified. (al) Allantois; (am) amnion; (ch) chorion; (ec) extraplacental cone; (exe) extraembryonic ectoderm; (hf) head fold; (pe&rm) parietal endoderm and Reichert's membrane; (ps) primitive streak; (ve) visceral endoderm; and (vys) visceral yolk sac. Bar, 250 μm. (F) E6.75 embryo sections were stained with anti-phosphohistone H3 antibody (pH3) and counterstained with propidium iodide (PI). (G) Numbers of pH3-positive cells in two wild-type (wt1 and wt2) and two Ecsit-/- mutant embryos (mut1 and mut2). (H) Whole-mount TUNEL staining of E6.75 embryos.