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. 2003 Dec 1;17(23):2950–2965. doi: 10.1101/gad.281203

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Figure 4. — Body muscles and ribs are perturbed in Pax3^{PAX3-FKHR-IRESnLacZ/+} embryos. (A,B) X-Gal staining of thoracic somites at E11.5 in Pax3^IRESnLacZ/+ (A) and Pax3^{PAX3-FKHR-IRESnLacZ/+} (B) embryos. Dispersed β-Gal⁺ cells remain adjacent to the somites in Pax3^{PAX3-FKHR-IRESnLacZ/+} embryos, and are particularly evident in the hypaxial region (B, black arrowhead). (C,D) The presence of the myogenic differentiation marker, α-actin, at E11.5 in Pax3^IRESnLacZ/+ (C) and Pax3^{PAX3-FKHR-IRESnLacZ/+} (D) embryos shows that dispersed cells that have undergone abnormal delamination are also terminally differentiated both epaxially (D, black arrowhead) and hypaxially (D, black arrow). In the Pax3^IRESnLacZ/+ control, α-actin-positive cells are confined to the myotome (C). (E,F) Expression of α-actin at E12.5 marks all the well-defined differentiated muscle masses of control Pax3^IRESnLacZ/+ embryos (E). In contrast, in Pax3^{PAX3-FKHR-IRESnLacZ/+} embryos (F), intercostal muscle masses are disorganized (F, black arrow; cf. E), and the segmental form of the rectus-abdominis muscle is no longer distinguishable (F, black arrowhead). (G,H) Alizarin red-Alcian blue staining of bone and cartilage at E18.5 shows distal rib fusions in Pax3^{PAX3-FKHR-IRESnLacZ/+} fetuses (H, arrowhead), which are not observed in the Pax3^IRESnLacZ/+ controls at the same stage (G, arrowhead). (FL) Forelimb; (HL) hindlimb.

Figure 4. — Body muscles and ribs are perturbed in Pax3^{PAX3-FKHR-IRESnLacZ/+} embryos. (A,B) X-Gal staining of thoracic somites at E11.5 in Pax3^IRESnLacZ/+ (A) and Pax3^{PAX3-FKHR-IRESnLacZ/+} (B) embryos. Dispersed β-Gal⁺ cells remain adjacent to the somites in Pax3^{PAX3-FKHR-IRESnLacZ/+} embryos, and are particularly evident in the hypaxial region (B, black arrowhead). (C,D) The presence of the myogenic differentiation marker, α-actin, at E11.5 in Pax3^IRESnLacZ/+ (C) and Pax3^{PAX3-FKHR-IRESnLacZ/+} (D) embryos shows that dispersed cells that have undergone abnormal delamination are also terminally differentiated both epaxially (D, black arrowhead) and hypaxially (D, black arrow). In the Pax3^IRESnLacZ/+ control, α-actin-positive cells are confined to the myotome (C). (E,F) Expression of α-actin at E12.5 marks all the well-defined differentiated muscle masses of control Pax3^IRESnLacZ/+ embryos (E). In contrast, in Pax3^{PAX3-FKHR-IRESnLacZ/+} embryos (F), intercostal muscle masses are disorganized (F, black arrow; cf. E), and the segmental form of the rectus-abdominis muscle is no longer distinguishable (F, black arrowhead). (G,H) Alizarin red-Alcian blue staining of bone and cartilage at E18.5 shows distal rib fusions in Pax3^{PAX3-FKHR-IRESnLacZ/+} fetuses (H, arrowhead), which are not observed in the Pax3^IRESnLacZ/+ controls at the same stage (G, arrowhead). (FL) Forelimb; (HL) hindlimb.