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. Author manuscript; available in PMC: 2010 Jun 24.
Published in final edited form as: Biochemistry. 2006 Oct 10;45(40):12216–12226. doi: 10.1021/bi0605235

Figure 3.

Figure 3

Assembly titration and kinetic assay data for CreWT and CreQQ reactions with LoxTA and LoxGC. (a) Substrate turnover as a function of active Cre4Lox2 complex concentration. Assembly titration data and averaged fit curves are shown for CreWT (black curves) and CreQQ (gray curves) with LoxTA (left plot) and LoxGC (right plot). Error bars denote the standard deviation from four replicate reactions. The inset details the CreWT/LoxGC reaction. LoxP and LoxAT reactions have been reported earlier (16). (b) Substrate turnover as a function of time. Time course data and fit curves are shown for CreWT (black curves) and CreQQ (gray curves) with LoxTA (left plot) and LoxGC (right plot). Error bars denote the standard deviation from four replicate reactions. The inset details the CreWT/LoxGC reaction.