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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Mol Biosyst. 2010 Mar 2;6(5):782–788. doi: 10.1039/b921912a

Figure 3.

Figure 3

BIX-01294 inhibited G9a and GLP methyltransferases in a mock screen of HMT activity in 9 plates for each enzyme. A, Dose-response curves are shown for BIX-01294 inhibition of G9a (squares) and GLP (triangles) enzymatic activity in the first (solid) and final (empty) plates of the mock screen. B, Reproducibility of IC50 value determinations for BIX-01294 across plates are shown for G9a (squares) and GLP (triangles). C, A counterscreen confirms BIX-01294 as a true HMT inhibitor as minimal loss of signal was observed in a b-rIgG binding assay. D, Reactive Blue 2 is a false positive in AlphaScreen assays and signal interference is observed in both HMT enzymatic reactions and the b-rIgG counterscreen. Data in (A) and (C) represent the mean and standard deviation of duplicate measurements.