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. 2000 Feb 15;105(4):469–478. doi: 10.1172/JCI6899

Figure 2.

Figure 2

Colonic PGE2 levels in 24-hour culture supernatants from either untreated mice (water controls) or WT, heterozygous, or homozygous mice for COX-1 or COX-2 deletion treated for 5 days with 10% DSS. The colons were removed, opened longitudinally, washed in PBS buffer containing penicillin, streptomycin, and fungizone (P/S/F, 100 U/mL) and kept in cold serum-free media (RPMI 1640 1× with P/S/F). The organs were cut into small pieces in a Petri dish containing fresh media and 100 mg of tissues were incubated at 37°C in 1 mL of fresh media for 24 hours. Culture supernatants were harvested and assayed for PGE2 secretion by RIA. Mean ± SEM, n = 6. *P < 0.05 versus water controls; +P < 0.05 versus COX-1+/–.