Figure 7.

Enhanced migration of macrophages (a and b) and smooth muscle cells (c) by MK. Values shown are means ± SD of migrated cell number observed in 10 fields at ×400. (a) MK was added at the indicated concentrations of 0–1,000 ng/mL to the assay well containing macrophages. (b) The filters in the assay well containing macrophages were coated on 1 side with MK at concentrations of 1–200 μg/mL. (c) MK at 100 ng/mL was added to the assay well containing smooth muscle cells. hMK, human MK produced in yeast. mMK, mouse MK produced by the baculovirus expression system (39). CM, conditioned medium of smooth muscle cells.