Table 1. Expression of CD40L and TRICOM molecules following infection of CLL cells with MVA-TRICOM or MVA-CD40L.
| CD40L % (MFI) | B7-1 % (MFI) | ICAM-1 % (MFI) | LFA-3 % (MFI) | |
|---|---|---|---|---|
| CLL 1 | ||||
| Uninfected | 10.3 (75) | 35.1 (49) | 88.9 (99) | 95.0 (243) |
| MVA-WT | 5.8 (58) | 13.2 (51) | 83.2 (105) | 93.6 (225) |
| MVA-TRICOM | 7.7 (156) | 83.1 (1712) | 91.7 (3432) | 90.7 (3063) |
| MVA-CD40L | 89.6 (3445) | 29.3 (72) | 82.6 (272) | 91.4 (339) |
| CLL 2 | ||||
| Uninfected | 15.3 (19) | 5.7 (19) | 76.2 (37) | 52.3 (47) |
| MVA-WT | 9.6 (25) | 5.2 (27) | 83.7 (33) | 68.6 (47) |
| MVA-TRICOM | 29.3 (26) | 64.8 (487) | 84.7 (798) | 80.6 (758) |
| MVA-CD40L | 54.1 (29) | 19.4 (25) | 72.0 (231) | 65.5 (85) |
| CLL 3 | ||||
| Uninfected | 3.6 (20) | 3.5 (20) | 21.6 (17) | 75.2 (38) |
| MVA-WT | 2.7 (12) | 3.4 (12) | 29.2 (11) | 79.8 (36) |
| MVA-TRICOM | 2.1 (70) | 75.8 (1285) | 72.0 (1059) | 83.2 (683) |
| MVA-CD40L | 81.6 (930) | 14.1 (45) | 36.1 (63) | 70.1 (53) |
| CLL 4 | ||||
| Uninfected | 4.6 (95) | 3.4 (21) | 38.6 (17) | 70.5 (49) |
| MVA-WT | 8.6 (15) | 8.3 (9) | 49.9 (14) | 83.5 (37) |
| MVA-TRICOM | 10.0 (11) | 86.4 (778) | 89.7 (1269) | 92.3 (945) |
| MVA-CD40L | 89.5 (774) | 25.1 (28) | 65.7 (53) | 72.8 (75) |
| CLL 5 | ||||
| Uninfected | 11.2 (71) | 5.5 (67) | 62.7 (127) | 79.5 (170) |
| MVA-WT | 12.8 (72) | 7.5 (57) | 41.6 (74) | 84.3 (152) |
| MVA-TRICOM | 13.0 (141) | 86.6 (1378) | 83.3 (3143) | 90.2 (2215) |
| MVA-CD40L | 89.9 (2010) | 16.1 (226) | 56.9 (228) | 86.6 (233) |
| CLL 6 | ||||
| Uninfected | 13.9 (25) | 17.8 (30) | 52.0 (69) | 85.7 (80) |
| MVA-WT | 21.5 (42) | 16.9 (42) | 62.4 (69) | 86.3 (70) |
| MVA-TRICOM | 28.3 (32) | 36.3 (674) | 86.6 (570) | 53.4 (40) |
| MVA-CD40L | 24.0 (50) | 25.8 (88) | 63.2 (165) | 88.9 (102) |
| CLL 7 | ||||
| Uninfected | 4.6 (60) | 4.2 (52) | 67.5 (20) | 67.4 (24) |
| MVA-WT | 5.2 (100) | 2.9 (40) | 71.3 (35) | 79.3 (29) |
| MVA-TRICOM | 3.3 (144) | 37.3 (868) | 67.3 (555) | 74.5 (250) |
| MVA-CD40L | 38.4 (79) | 15.3 (41) | 74.3 (24) | 76.1 (29) |
| CLL 8 | ||||
| Uninfected | 7.0 (45) | 1.7 (36) | 93.1 (89) | 93.3 (102) |
| MVA-WT | 1.4 (68) | 2.6 (55) | 87.8 (92) | 87.2 (88) |
| MVA-TRICOM | 0.5 (54) | 68.7 (1133) | 85.7 (3474) | 82.7 (2428) |
| MVA-CD40L | 91.7 (1193) | 91.4 (482) | 92.9 (1196) | 89.2 (687) |
| CLL 9 | ||||
| Uninfected | 4.5 (19) | 4.6 (20) | 86.3 (50) | 81.4 (73) |
| MVA-WT | 5.1 (38) | 4.5 (20) | 84.4 (51) | 61.9 (60) |
| MVA-TRICOM | 16.3 (21) | 74.3 (454) | 92.2 (1443) | 88.9 (1002) |
| MVA-CD40L | 69.3 (141) | 31.1 (122) | 85.3 (226) | 69.4 (176) |
| CLL 10 | ||||
| Uninfected | 7.2 (53) | 2.6 (53) | 47.8 (26) | 57.6 (39) |
| MVA-WT | 11.8 (71) | 3.2 (47) | 59.3 (32) | 68.6 (40) |
| MVA-TRICOM | 12.4 (51) | 48.3 (296) | 77.2 (572) | 77.1 (486) |
| MVA-CD40L | 32.8 (35) | 37.1 (47) | 85.6 (124) | 86.8 (97) |
| CLL 11 | ||||
| Uninfected | 12.3 (32) | 8.4 (20) | 56.0 (39) | 74.2 (48) |
| MVA-WT | 15.0 (83) | 9.3 (27) | 60.5 (40) | 67.4 (51) |
| MVA-TRICOM | 22.0 (74) | 28.6 (209) | 59.0 (242) | 64.9 (210) |
| MVA-CD40L | 16.9 (136) | 9.1 (53) | 43.2 (79) | 51.3 (66) |
| CLL 12 | ||||
| Uninfected | 9.9 (37) | 9.8 (17) | 70.9 (22) | 81.9 (44) |
| MVA-WT | 6.5 (21) | 44.2 (14) | 64.6 (25) | 85.3 (31) |
| MVA-TRICOM | 7.7 (26) | 79.0 (263) | 96.4 (667) | 85.6 (418) |
| MVA-CD40L | 78.1 (352) | 12.4 (40) | 46.5 (50) | 80.3 (40) |
CLL cells were infected with the indicated MVA vectors. Flow cytometry analysis was performed 24 hours following infection to determine expression of CD40L and TRICOM molecules by the CLL cells. The percentage and MFI of expression of these molecules by CD19+ CLL cells is shown.