Figure 4. Model predicted flux values in central and C1 metabolism.

The figure shows the range of flux values calculated using FVA that correspond to maximal biomass yields in lactate-limited aerobic growth when malate synthase, pyruvate formate lyase, NAD+ dependent isocitrate dehydrogenase, and fumarate reductase are constrained to be zero (see text for details). Flux values are reported as the percentage of the lactate consumption rate, 4.11 mmol/(g AFDW•h). Cellular growth rate was constrained to 0.085 h⁻¹. Metabolite abbreviations are described in text and/or can be found in Table S3.