Skip to main content
. 2000 Feb 15;105(4):497–504. doi: 10.1172/JCI8541

Figure 3.

Figure 3

Lipid IVa and RSLA stimulate the release of the cytokine IL-6 from CD14-expressing hamster fibroblasts but become LPS antagonists when these cells overexpress human TLR4. CHO/CD14 cells and CHO/CD14/huTLR4 cells were allowed to adhere overnight in 24-well dishes. The next day, the cells were exposed to increasing amounts of S. minnesota Re595 LPS in the presence of medium only (diamonds), 0.5 μg/mL lipid IVa or RSLA (squares), or 5 μg/mL of the same compounds (circles) for 10 hours. Supernatants were assayed for IL-6 by bioassay. Data represents mean values of 3 seperate fractions ± SD shown is 1 experiment out of 2 performed.