Table 1.
Cross-species amplification of three-spined stickleback SSR primers in nine-spined sticklebacks.
| SSR type | Primer site | N | Amplified | Polymorphic |
|---|---|---|---|---|
| Gene-based | All | 158 | 27 (17.1%) | 16 (10.1%) |
| Exonic pair | 9 | 7 (77.8%) | 4 (44.4%) | |
| Intronic pair | 53 | 2 (3.8%) | 1 (1.9%) | |
| Intergenic pair | 55 | 9 (16.4%) | 7 (12.7%) | |
| Other combinations | 41 | 9 (22.0%) | 4 (9.8%) | |
| Genomic | All | 101 | 35 (34.7%) | 26 (25.7%) |
| Exonic pair | 1 | 1 (100%) | 1 (100%) | |
| Intronic pair | 34 | 14 (41.2%) | 10 (29.4%) | |
| Intergenic pair | 58 | 16 (27.6%) | 11 (19.0%) | |
| Other combinations | 6 | 3 (50.0%) | 3 (50.0%) | |
| Unknown | 2 | 1 (50.0%) | 1 (50.0%) | |
| EST-derived | All | 87 | 35 (40.2%) | 21 (24.1%) |
| Exonic pair | 14 | 7 (50.0%) | 6 (42.9%) | |
| Intronic pair | 11 | 4 (36.4%) | 1 (9.1%) | |
| Intergenic pair | 47 | 17 (36.2%) | 10 (21.3%) | |
| Other combinations | 15 | 7 (46.7%) | 4 (26.7%) |