Table 2.
SSR markers for functionally important genes in nine-spined sticklebacks and their polymorphism (P) in three populations.
| Gene ID | SSR marker | GenBank accession no | |||||
|---|---|---|---|---|---|---|---|
| Locus | Repeat motif | Forward primer (5'-3') | Reverse primer (5'-3') | Ta (°C) | P | ||
| ACAPRa | Ppgm1 | (CTC)12 | AGCTGCCATTTTAAATCCTCCTC | CTCACCATGATGGAAGCCAC | 53 | No | GU553378 |
| ACAPRb | Ppgm2 | (AACT)5 | GGTCTGCCAGGTCATTTCTC | AACGGCACTCATCTGGTTAGT | 53 | Yes | GU553379 |
| AE1 | Ppgm3 | (GA)8GG(GA)5 | AACACATGACATCACTGCAGC | ACAGGTAAGTCAGTTGTTTCAGG | 53 | Yes | GU553380 |
| AQP9 | Ppgm4 | (TGT)6 | AGAAAATAAGCAGCCGTAGC | TGCACGTAAATGGTCTGATT | 55 | No | GU553381 |
| ATP1A1 | Ppgm5 | (TG)6 | CCATAGGACGATCACAAG | GAATGAAGTCTTTGTTGTGGGTC | 53 | No | GU553382 |
| Ppgm6 | (CA)5 | AGCAGAGCAAAGAACAGGACTC | GATCTCTTTTGCTCTGGAGTTGG | 53 | No | GU553382 | |
| ATP1A2 | Ppgm7 | (TG)5/(GT)12 | TGCATAATGGTCCCCCGTG | AGGCCTTGGCATCCCTG | 53 | Yes | GU553383 |
| ATP4A | Ppgm8 | (TC)5 | TCATTGTAATTTCCGCCTTT | TTTCATCACCAACAGGTAGC | 55 | Yes | GU553384 |
| ATP6V1Aa | Ppgm9 | (TG)6/(TG)10 | GACCGATTTCATCTCTGGAC | TGACTCTTTTCCCTCCACTT | 55 | Yes | GU553385 |
| ATP6V1Ab | Ppgm10 | (CA)6 | GCAGGATACCCGCTGTCT | AAGTTCACAAAGGATGCACA | 55 | Yes | GU553386 |
| CFTR | Ppgm11 | (CA)13 | CACTGCTAACACACATCAGC | AAGCGATACCCATCTGTCC | 55 | Yes | GU553387 |
| CLCN3 | Ppgm12 | (CA)11 | AGTCGGCATGGGAGTTCAC | GCGATGTCAATCAGGCCG | 53 | Yes | GU553388 |
| CLCN4 | Ppgm13 | (CTC)5 | GTTTGAATCCCACAACTTCA | ACTACGTCAACAACCCCAAC | 55 | No | GU553389 |
| CLCN7 | Ppgm14 | (TG)10 | CGCTCTGAACAGCTTAAACA | ACGAGAGGGAGTGCATGA | 55 | Yes | GU553390 |
| CLCNK | Ppgm15 | (TA)4(CA)(TA) | GGCCACCTAGAATTGATGAC | TCAGAGTCCAAACACGAGAA | 55 | No | GU553391 |
| CSP2 | Ppgm16 | (AAAT)3 | CGTCGAACTCTACAACCTCC | AGACGTGTTTGTTCATCAGG | 55 | Yes | GU553392 |
| DIO1 | Ppgm17 | (TC)9 | CAATCAGGATGTCCAACCA | GCAGCATGGGATGAGAAC | 55 | Yes | GU553393 |
| eEF1A1b | Ppgm18 | (AC)9 | CACTACAGAGTCTAGTCTGAG | TCTTCAGTTAAATGAACCGGTTGC | 55 | Yes | GU553394 |
| FERH1 | Ppgm19 | (ATC)5 | TTCTCTCGTTTCTCCAGAGC | GAAATTGACACTGCTGGTTG | 55 | Yes | GU553395 |
| FGF6a | Ppgm20 | C12 | CATCCTTCACCCCAATCTTA | TCTGTCCCCTCTTTCAATGT | 55 | Yes | GU553396 |
| FGF18 | Ppgm21 | (AG)12 | TGCCTACTCACACCCACTAA | ATGAGAAATCAATGGAGGGA | 55 | Yes | GU553397 |
| GH | Ppgm22 | (GTATA)3 | TGCGTGGTGTAGTATAGTGTAGTC | AGAGCAACGTCAACTCAACA | 55 | No | GU553398 |
| GHRH | Ppgm23 | (AT)8 | AAGATGAGTTTCCCGCTCTA | TTATTGACTTGACCCTTGACC | 55 | No | GU553399 |
| GHR-1 | Ppgm24 | (AC)7 | CCACTACCTCTGCCCTAAAA | TTTCCTTTGGCTTCAATCTC | 55 | Yes | GU553400 |
| GHR-2 | Ppgm25 | (TA)6 | TCAACTCTGACCTTCTTGAGG | ACCACAGGTTCACCAAAGAT | 55 | Yes | GU553401 |
| GR1 | Ppgm26 | A8 | CTGGTACTGTCCTGATGGAG | TTCTCATAACCACAACTGGC | 55 | Yes | GU553402 |
| GR2 | Ppgm27 | A8 | AGAGCACGACAAAACACAGA | AGCAGAAATTGAACAGCACA | 55 | No | GU553403 |
| GTF2B | Ppgm28 | (AC)4 | TGTAATCCCAATACGACGC | AGTATCTGAACCCGCACATT | 55 | Yes | GU553404 |
| HPX | Ppgm29 | (AC)26 | GTGCTTTTAGAAAGACCACCG | TATTACTCTATAGCCGGCAGC | 55 | Yes | GU553405 |
| HSP25 | Ppgm30 | (AC)13 | GCAGCGTACATTCTGTTCAAC | GGTTTCTTATGTGGGTGTGAC | 53 | Yes | GU553406 |
| HSP47a | Ppgm31 | (CA)6 | TGCATCATCTGCACTGAAACG | GGGGCAATGATCGTCAATG | 55 | Yes | GU553407 |
| HSP70Aa | Ppgm32 | (AC)15 | CAAAGACCTGCACACACATT | GGGAGCTGTCGATACGTTTA | 55 | Yes | GU553408 |
| HSP70Ab | Ppgm33 | (AC)7 | ATCTACAGGGATACCACAGTAC | TGTTTACTCCGGTCAATGAAACC | 53 | No | GU553409 |
| HSP70Ac | Ppgm34 | (TG)5 | TACTGTTCCACTTGCCCATT | TCACAACTCAGGATCTCGAA | 55 | No | GU553410 |
| HSP70B | Ppgm35 | (TC)14 | TGAGGGTAAAAGCTGTAGCA | ATTATCCCAGAACACTCCCA | 55 | Yes | GU553411 |
| HSP90Ab | Ppgm36 | (CA)5 | GTCAAACCGGACATTAGGAC | CAGACGTGAAACTACGCTTG | 55 | Yes | GU553412 |
| HSP90B | Ppgm37 | (TCG)4 | AGTTATGAAGAAACCGCGTC | GTGATGGCTGTAGCTTGTTG | 55 | Yes | GU553413 |
| IGF-I | Ppgm38 | (CGC)4 | AAGGACGAGCTCGGCTAC | AGGATGCGGCTGCAGATG | 53 | No | GU553414 |
| IGF-II | Ppgm39 | (TA)13/(TA)5 | GTTAGGCTTTTACTTGGGTTTCC | TCATTACGCAAGATACAGCTCAG | 53 | Yes | GU553415 |
| Kir2.1a | Ppgm40 | (TA)10 | CTGGAATGACACCAGCCAG | AGTCAGCTCCAAGCTTGTG | 53 | Yes | GU553416 |
| Kir2.1c | Ppgm41 | (TG)7 | CAAAGTGAGCAACAGTGAGC | AAAGGTCGAGGAAGATGATG | 55 | No | GU553417 |
| Kir2.2 | Ppgm42 | (TC)12 | ACAGGTACGAAGCGTTTAGC | AGAGCAAAGAAATAGACGGG | 55 | Yes | GU553418 |
| MSTNa | Ppgm43 | (CA)5 | AGTCCGCAATAAGCTCAAAC | ACAAAGCCGTCTAGGTGTTC | 55 | No | GU553419 |
| MSTNb | Ppgm44 | (GGA)8 | GCTGGAGCAGTACGACC | GGTGATGATGGTCTCCGT | 55 | No | GU553420 |
| MYHa | Ppgm45 | (TA)3C(TA)3 | AAAGCTCCAAGTAACGCTGT | ACTTTTGTTTCCAATCTGCC | 55 | No | GU553421 |
| MYHe | Ppgm46 | (GTAA)4 | ATGTTAATTGCTTTGTGCGA | TGAAACACAGGAGCTTGAGA | 55 | Yes | GU553422 |
| NKCC1b | Ppgm47 | (CA)13 | ATATGTTCAGCACGCAGCG | GTCAAAGGAGTCTTAGTGAGTG | 55 | Yes | GU553423 |
| NPY2Rb | Ppgm48 | (GT)26 | CACTCAGGAGAAGTGAGGC | GGAACGATTACAGGTACGGAC | 53 | Yes | GU553424 |
| NPYP | Ppgm49 | T10 | CTGCAGCGGACGGGATTAG | AACACAGGACCGACTTTGAGG | 53 | No | GU553425 |
| PKMa | Ppgm50 | (GA)5 | GTGGTACTGCTGGTTGTACT | AAGGTCAAACGGCGTCGC | 53 | Yes | GU553426 |
| PVALBb | Ppgm51 | (CA)6 | AGCTGAACTTTGGTGTGTCTC | GTTGATGTGCATTTATGGGA | 55 | Yes | GU553427 |
| SHH | Ppgm52 | G18 | AGAAACGTGGTTATTGAGGC | CCTGCTCTTTATTGGGTTTT | 55 | Yes | GU553428 |
| SLC14 | Ppgm53 | (TG)13 | CCATCATCTCTACACATAATCAAAC | CACTGATTACAGATTGTGTGCTG | 53 | Yes | GU553429 |
| SSR1b | Ppgm54 | (TG)12 | TGTCCTCGCAAAGTTCATAA | TGACCGAGCATTTTACTTGA | 55 | Yes | GU553430 |
| T1R3 | Ppgm55 | T18 | ATGCACTGCGTTATCACTCC | GCTTTGTTTAACGTCAGTATTTCG | 55 | Yes | GU553431 |
| TAAR | Ppgm56 | (AC)12 | CAAGGACCACGCTAAAGGTA | GATTTCTTCTTGATGTCCGC | 55 | Yes | GU553432 |
| TBX4 | Ppgm57 | (GT)8 | GAGTACGAGCAGGTTTGGTT | GTAACACACACGGTTTTGGA | 55 | Yes | GU553433 |
| TTP | Ppgm58 | (AC)3(TC)(AC)3 | GCCACTTATACATGCTCGC | GATTCTCCCTGCCTCACA | 55 | Yes | GU553434 |
Ta, annealing temperature.