Fig 5.

Effect of adipocyte-secreted factors on Runx2 nuclear-binding activity in human osteoblasts: Runx2 DNA-binding activity was determined using ELISA-based Runx2 activation kit and quantified by colorimetry. Osteoblasts exposed to supernatants obtained from adipocytes as well as treated with stearate and palmitate showed a significantly lower activity of the Runx2 nuclear complex in the nuclei as compared with controls. This effect was reverted by addition of cerulenin into the adipogenic media. Finally, addition of cerulenin into osteogenic media did not show a direct effect on the complex in normal osteoblasts. Values are mean ± S.E.M. of six wells per group in three independent experiments; *P < 0.001 cerulenin treated versus matched untreated cells. δP < 0.01 for cells exposed to adipogenic factors versus control (-/OB).