Table 1.

PCR primers used for bisulfite sequencing and methylation specific PCR

	Primer sequence	Product size
Bisulfite sequencing
Region1 Forward	5'-TAGTTTTTTGTTTAAAGGGTTTTAACGGGT-3'	365 bp
Region1 Reverse	5'- ACCTAAACTTAATAACTCCCCCTCA -3'
Region2 Forward	5'- ATTTTGGTATATTTTTGGGGAGTTT-3'	360 bp
Region2 Reverse	5'- CCCAAACACAAAATCTCATTTCTA -3'
Methylation specific PCR
Methylated Forward	5'-ATTTTTCGTCGGGAGTGGTC-3'	79 bp
Methylated Reverse	5'-GCAACGAATACGACGAACCT-3'
Unmethylated Forward	5'-GGGAGTGGTTGTGTGGTTTT-3'	103 bp
Unmethylated Reverse	5'-AACTTGGGGACACCCAAA-3'