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. 2010 Jun 8;10:265. doi: 10.1186/1471-2407-10-265

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Copyright ©2010 Andreu-Pérez et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Methylthioadenosine (MTA) decreases melanoma cell viability. Clonogenic assays of 37-31E, MeWo, SKMel147, SKMel103, UACC903 and Colo 829 melanoma cells in complete medium with increasing concentrations of Methylthioadenosine (MTA). Three hundred cells were seeded clones were visualized by crystal violet staining and counted. Fresh media was changed every 48 h. Graphs represent the percentage reduction in the number of clones upon different treatments. p-value was calculated performing a Wilcoxon-signed rank test (Vassar Stats).