Fig 2.

(A) PEITC reduces the steady state levels of ER-α66 protein in H3396 cells. H3396 cells were treated with vehicle (DMSO), 5 μM PEITC or 5 μM ICI 182, 780, together with MG132 10 μM or MG132 10 μM alone for 12 hrs and lysed for Western blot analysis with anti-ER-α66 and anti-β-actin antibodies. (B) Effects of PEITC on the mRNA levels of ER-α66 in H3396 cells. H3396 cells were treated with vehicle, 2.5, 5 or 10 μM of PEITC for 12 hrs, total RNA were extracted and 1 μg of total RNA were used for RT-PCR using specific primers for ER-α66 and β-actin as described in methods. RT-PCR products were separated by 1.5% agarose gels and stained with ethidium bromide. Experiments were repeated three times, and the representative results are shown.