PEITC reduces the steady state levels of ER-α36 protein. MCF7 (A) and H3396 (B) cells were treated with vehicle (DMSO), 1, 2.5, 5 and 10 μM of PEITC or ICI 182780 for 12 hrs and lysed for Western blot analysis with anti-ER-α36 and anti-β-actin antibodies. MCF7 (C) and H3396 (D) cells were treated with 5 μM of PEITC or ICI 182, 780 for indicated time-points, and lysed for Western blot analysis with anti-ER-α36 and anti-β-actin antibodies. Experiments were repeated three times, and the representative results are shown.