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The proteasome inhibitor MG132 restored levels of ER-α36 protein reduced by PEITC in MCF7 and H3396 cells. MCF7 (A) and H3396 (B) cells were incubated for 12 hrs in the vehicle (DMSO), 5 μM PEITC with or without MG 132 (10 μM), MG132 10 μM alone and lysed for Western blot analysis with anti-ER-α36 and anti-β-actin antibodies. Experiments were repeated three times, and the representative results are shown.
