Fig 6.

PEITC and ICI 182, 780 failed to influence the levels of ER-α36 mRNA. MCF7 and H3396 cells were incubated for 12 hrs in vehicle (DMSO) or 1, 2.5, 5 and 10 μM of PEITC or ICI 182, 780. Total RNA were extracted and 1 μg of total RNA were used for semi-quantitative RT-PCR using specific primers for ER-α36 and β-actin as described in methods. RT-PCR products were separated by 1.5% agarose gels and stained with ethidium bromide. (A) MCF7 cells treated by PEITC, (B) H3396 cells treated by PEITC, (C) MCF7 cells treated by ICI 182, 780, (D) H3396 cells treated by ICI 182, 780. Experiments were repeated three times, and the representative results were shown in the figure.