Fig 7.

(A) PEITC inhibits the proliferation of ER^– breast cancer MDA-MB-231 and SK-BR-3 cells. MDA-MB-231 and SK-BR-3 cells in oestrogen-free medium were treated with vehicle, 1, 2.5, 5 or 10 μM of PEITC or ICI 182, 780 for 6 days and then counted with a haemocytometer. Experiments were repeated three times, and the means ± S.E. are shown. (B) PEITC reduces the steady state levels of ER-α36 protein in MDA-MB-231 and SK-BR-3 cells. MDA-MB-231 and SK-BR-3 cells were treated with vehicle (DMSO), 1, 2.5, 5 and 10 μM of PEITC or ICI 182780 for 12 hrs and lysed for Western blot analysis with anti-ER-α36 and anti-β-actin antibodies. Experiments were repeated three times, and the representative results are shown.