Fig 8.

PEITC at low concentration induces ERK phosphorylation in ER-α36 expressing cells. HEK293/V and HEK293/36 cells were cultured in serum-free medium for 24 hrs and treated with vehicle (DMSO) or 0.1 pM, 10 pM, 1 nM, 100 nM or 1 μM of PEITC for 30 min. HEK293/V cells were also treated by EGF 10 ng/ml as positive control. Cells were then harvested and lysed for Western blot analysis with anti-phospho-p44/42 ERK and anti-p44/42 ERK antibodies. Experiments were repeated three times, and the representative result is shown.