Table 1.
A collection of X-linked insertion mutations associated with altered sensitivity to halothane
| Strain name | Location of insert |
Putatively affected gene(s) |
Potency ratio (95% CL) |
% Shift in EC50 |
|---|---|---|---|---|
| JC01 | 48,375 | CG17707 | 2.03 (1.83-2.27) | -50.8 |
| JC02 | 1,762,806 | CG14815 & CG14803 | 1.55 (1.41-1.72) | -35.7 |
| JC03 | 1,767,558 | CG14816 & CG14804 | 1.55 (1.40-1.71) | -35.3 |
| JC04 | 2,411,672 | trol | 1.89 (1.70-2.09) | -47.0 |
| JC05 | 2,536,688 | sgg | 1.55 (1.40-1.71) | -35.3 |
| JC06 | 10,184,177 | alpha-Man-I | 1.62 (1.47-1.79) | -38.3 |
| JC07 | 10,184,200 | alpha-Man-I | 1.37 (1.25-1.49) | -26.8 |
| JC08 | 10,184,223 | alpha-Man-I | 1.66 (1.50-1.84) | -39.8 |
| JC09 | 11,623,218 | inaF | 0.88 (0.80-0.96) | +14.2 |
| JC10 | 13,890,439 | ben | 1.98 (1.79-2.21) | -49.6 |
| JC11 | 18,428,614 | Bx | 2.20 (1.97-2.46) | -54.5 |
| JC12 | 18,428,636 | Bx | 1.89 (1.71-2.10) | -47.1 |
| JC13 | 18,428,677 | Bx | 1.41 (1.29-1.54) | -29.0 |
| JC14 | 19,781,155 | amn & CG32529 | 1.11 (1.03-1.22) | -10.6 |
The position of each insert is given using the coordinates of the complete sequence of the Drosophila melanogaster X chromosome (Accession NC_004354.3). The annotation of the Drosophila genome (Release 5.13), which is is available through FlyBase < http://flybase.org/cgi-bin/gbrowse/dmel/>, was used to identify genes that are likely to be affected by the listed insertions because the latter either disrupt the transcription unit of the named gene or lie within a few kb of its 5′-end. The final two columns present related metrics for the halothane sensitivity of each strain relative to that of the Canton-S control strain. The potency ratio is defined as EC50 (control strain)/EC50 (mutant strain). This ratio is calculated from a logistic fit of concentration-response curves and is presented together with 95% confidence limits. The shift in EC50 is defined as 100 * [EC50 (mutant strain)-EC50 (control strain)]/ EC50 (control strain)]; it is calculated from the potency ratio R by the formula: 100* [(1/R)-1]. The absolute value of the EC50 for each mutant line can be calculated from these metrics and the halothane EC50 value determined from the concentration-response curve for the Canton-S control strain: 0.26 (v/v%).