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. Author manuscript; available in PMC: 2010 Sep 22.
Published in final edited form as: Mol Microbiol. 2009 May;72(3):763–778. doi: 10.1111/j.1365-2958.2009.06684.x

Figure 8. HrtAB expression in an animal model of cutaneous anthrax.

Figure 8

A. Hemin-dependent luminescence of B. anthracis harboring phrt.Lux. B. anthracis harboring promoterless pXen-1 or pXen-1 with the Lux genes under the control of the hrtAB promoter (phrt.Lux) were grown on BHI agar containing no hemin, BHI + 1 µM hemin, or BHI + 10 µM hemin. Plates were visualized by IVIS. B. Activation of the hrtAB promoter during anthrax infection. Spores of B. anthracis harboring pXen-1 or phrt.Lux were used to infect groups of five A/J mice via a sub-dermal injection in the left inguinal region. Luminescence of B. anthracis was tracked every 12 hours post infection (p.i.) by IVIS. Shown is one mouse representative of each group.