Table 1.
Effects of resveratrol on CSE-stimulated caspase activity
Cas 9 | Without DTT |
With DTT |
||
---|---|---|---|---|
4h | 24h | 4h | 24h | |
0 | 1±0.05 | 1.23±0.19 | 1.55±0.08 | 1.55±0.23 |
R | 0.82±0.13 | 1.46±0.21 | 1.46±0.09 | 2.24±0.33 |
10% | 1.77±0.15* | 3.23±0.32* | 4.1±0.33* | 5.04±0.42* |
10%+R | 2.31±0.25*# | 4.26±0.28*# | 4.99±0.29*# | 6.1±0.35*# |
20% | 3.87±0.42* | 5.74±0.42* | 6.29±0.52* | 8.33±0.31* |
20%+R | 5.21±0.38*# | 7.25±0.58*# | 8.64±0.46*# | 9.51±0.49*# |
Cas 3 | ||||
0 | 1±0.05 | 1.04±0.23 | 1.74±0.09 | 2.25±0.31 |
R | 0.81±0.07 | 1.72±0.37 | 1.61±0.06 | 2.42±0.16 |
10% | 1.88±0.21* | 23.13±3.6* | 5.76±0.8* | 44.21±5.18* |
10%+R | 2.61±0.3*# | 41.39±5.71*# | 8.12±0.71*# | 58.81±4.32*# |
20% | 4.23±0.59* | 34.63±3.42* | 7.26±0.77* | 52.88±6.71* |
20%+R | 5.69±0.42*# | 53.24±8.69*# | 9.97±1.26*# | 78.17±5.25*# |
HBE1 cells were pretreated with 5 μM resveratrol for 24 h before being treated with/without CSE for 4 and 24 h; and the caspase activity was measured as described in Methods. Values are mean ± SE of three experiments.
P<0.05 compared with vehicle control (ethanol) of 0% CSE treatment;
P<0.05 compared with vehicle control (ethanol) of same CSE exposure. Cas 3, caspase 3; cas 9, caspase 9; R, resveratrol; 0, vehicle control (ethanol).