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Sequence analysis of the mutant and wild-type γ-subunit cDNA. RNA was isolated from cultured fibroblasts, reverse transcribed, and a 300-bp portion of the γ-subunit cDNA amplified by PCR as described in Methods. The amplified fragment was resolved by agarose gel electrophoresis, excised, and directly sequenced using the PCR primers as primers and fluorescent terminators. (a) Wild-type sequence. (b) Mutant sequence. (c) Effect of the insertion on the γ-subunit protein.
