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Linkage of the γ-subunit mutation to MLIII in families 2 and 3 by SSCP analysis. A 680-bp region of the γ-subunit gene containing the γ-subunit mutation was amplified, denatured, electrophoresed, and detected by autoradiography as described in Methods. The SSCP pattern of each patient tested is given below the pedigree symbol. (a) Family 2; (b) family 3. m, mutant allele; +, wild-type allele.
