Skip to main content
. 2000 Mar 1;105(5):567–575. doi: 10.1172/JCI7497

Figure 6.

Figure 6

Effect of HDGF overexpression in SMCs on BrdU uptake and cell localization. SMCs were transfected with HA-epitope–tagged HDGF (a–c) or empty vector (d–f) and pulsed with BrdU as a marker of DNA synthesis for 12 hours. Cells were stained with an HA mAb and an FITC-conjugated secondary antibody to detect HDGF-transfected cells (green, a and d) and an alkaline phosphatase–conjugated anti-BrdU antibody to detect cells replicating their DNA (red, b and e). c is an overlay of a and b demonstrating colocalization (yellow) of HDGF to the nucleus of cells replicating their DNA. f is an overlay of d and e.