Table 1.

Primer used for conventional PCR and results from PCR analysis of four S. aureus strains.

Primer name1	Nucleotide sequence (5' → 3')	Primer location2	Annealing temperature (°C)	PCR results
				Mu50	MW2	Newman	SA45
a forward	TAT TCA TTG CCC TAA CGT T	789421	49	+	+	-	+
a reverse	CCG TCT AGC CAT AAA TTG ATC	789842
b forward	TAT TCA TTG CCC TAA CGT G	783956	51	-	-	+	-
b reverse	CCG TCT AGC CAT AAA TTG ATT	784377
c forward	GGC AAG ATG GTT ATC ATG	789043	47	+	+	-	+
c reverse	CGA TTA TTA TCA TGT AAC G	789799
d forward	GTT CTG ATG AGA ACT ATG	781925	48	-	-	+	-
d reverse	CGT CTC CGC AAT TTT C	782948
e forward	GGC TAT AGA TGG ATT AC	793236	47	+	+	-	+
e reverse	AGA GCT TCG TCA ATT TCA	794180
f forward	GGT AGA CAA GGC AGG TAA TAG	787832	55	-	-	+	-
f reverse	GTG GAC TTC CTA CAA CGC	788235
g forward	CAT TGA ATG GTT AGT TGT AC	761697	50	-	+	-	+
g reverse	GTC CAA GTT ATA CAT TAT CGG	762676
h forward	GAA CGC GTC TAT AGA AAA G	782755	51	+	-	-	-
h reverse	GTC CAA GTT ATA CAT TAT CGG	783832

(+) amplification occurred in PCR using the primer pair and genomic DNA from the S. aureus strain listed. (-) no PCR amplification was observed.

¹Primer names indicate the physical position of PCR amplicon in Figure 6.

²Primer location indicates the position of the first 5'-nucleotide within the annotated genomes.