Table 2.
Sequences and fluorescent dyes for primers and hybridization probes used for real-time PCR.
| Target | Primer/probe | Nucleotide sequence (5' → 3') |
|---|---|---|
| sea | ESA-1 | ACG ATC AAT TTT TAC AGC |
| ToxA reverse | CCG AAG GTT CTG TAG AAG T | |
| ToxA-Fluo1 | CCT TTG GAA ACG GTT AAA ACG AAT AAG AAA-FL1 | |
| ToxA-Red1 | LC-R640-TGT AAC TGT TCA GGA GTT GGA TCT TCA-p2 | |
| rrn | rRNA forward | TGT CGT GAG ATG TTG GG |
| rRNA reverse | ACT AGC GAT TCC AGC TT | |
| Probe 1 | GGA CAA TAC AAA GGG CAG CG-FL | |
| Probe 2 | LC-R705-ACC GCG AGG TCA AGC A-p3 |
1The donor probe is labeled with fluorescein (FL) at the 3" end.
2The acceptor probe is labeled with LC Red640 (LC-R640) at the 5" end and the 3" hydroxy group is phosphorylated (p).
3The acceptor probe is labeled with LC Red705 (LC-R705) at the 5" end and the 3" hydroxy group is phosphorylated (p).