Figure 3.
Modulation of colon and small bowel epithelial cell permeability by miR-29a. FHC colonic cells and FHs74Int small intestinal epithelial cells were transfected with either100 nmol/L miR-29a–specific miRNA precursors / inhibitors or control miRNA precursors / inhibitors. Permeability was assessed after 48 hours using In Vitro Permeability Assay Kits. Over-expression of miR-29a significantly enhanced epithelial permeability in FHC (Panel A) and FHs74Int (Panel B) cells whereas silencing miR-29a had the opposite effect (*p < 0.05, ** p< 0.01 when compared with controls).