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. Author manuscript; available in PMC: 2011 Jun 1.

Published in final edited form as: Gut. 2009 Dec 1;59(6):775–784. doi: 10.1136/gut.2009.181834

miR-29a modulates expression of glutamine synthetase (GS). Small bowel tissue (Panel A) and colon tissue (Panel B) homogenates were obtained from 8 IBS patients with increased intestinal membrane permeability as well as 8 normal controls. Glutamine synthetase expression was significantly reduced in all 8 IBS patients compared with normal controls. Panel C: FHC human colon and FHs74Int small bowel epithelial cells were transfected with 100 nmol/L miR-29a control or precursors. Cell lysates were obtained after 72 hours for immunoblot analysis of glutamine synthetase expression. Up-regulation of miR-29a significantly silenced glutamine synthetase expression in human colon and intestinal epithelial cells. Panels D & E: Human FHC colon and human FHs74Int small bowel epithelial cells were transfected with 100 nmol/L miR-29a precursor or control construct. Immunocyto-chemistry for glutamine synthetase (GS) was done after 72 hours. A decrease in glutamine synthetase expression was observed in both types of epithelial cells transfected with miR-29a precursor (lower panels) compared with control precursor (upper panels).

miR-29a modulates expression of glutamine synthetase (GS). Small bowel tissue (Panel A) and colon tissue (Panel B) homogenates were obtained from 8 IBS patients with increased intestinal membrane permeability as well as 8 normal controls. Glutamine synthetase expression was significantly reduced in all 8 IBS patients compared with normal controls. Panel C: FHC human colon and FHs74Int small bowel epithelial cells were transfected with 100 nmol/L miR-29a control or precursors. Cell lysates were obtained after 72 hours for immunoblot analysis of glutamine synthetase expression. Up-regulation of miR-29a significantly silenced glutamine synthetase expression in human colon and intestinal epithelial cells. Panels D & E: Human FHC colon and human FHs74Int small bowel epithelial cells were transfected with 100 nmol/L miR-29a precursor or control construct. Immunocyto-chemistry for glutamine synthetase (GS) was done after 72 hours. A decrease in glutamine synthetase expression was observed in both types of epithelial cells transfected with miR-29a precursor (lower panels) compared with control precursor (upper panels).