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. 2010 Jun 24;6(6):e1000957. doi: 10.1371/journal.ppat.1000957

Figure 5. ESX1 and EspA activity are required for the ability of Mtb to survive cell wall stress.

Figure 5

A. Bacterial survival after SDS stress. The indicated strains were exposed to increasing concentrations of SDS for 6 hours and then plated for CFU. Untreated bacteria were plated as a control and survival is shown. The mean+/−standard deviations of three independent biologic replicates are expressed. Strains lacking ESX1 and espACD were significantly more susceptible to SDS stress than H37Rv as assessed by T-test (***p<0.005). B. The indicated strains were exposed to treatment medium containing 0.1% SDS or control medium for 6 hours and then plated for CFU. Strains carrying tetracycline inducible vector and paired control strained were cultured in AT at 100 ng/ml overnight prior to and during SDS treatment. The mean+/−standard deviations of three independent biologic replicates are expressed. Data are representative of at least three fully independent experiments. Strains lacking ESX1 function, espACD and EspA disulfide bond formation were significantly more susceptible to SDS stress than H37Rv as determined by T-test (for these comparisons, all p<0.01). C. Bacterial survival after n-dodecyl beta-D-maltoside or TritonX-100 exposure. Bacterial metabolism as measured by Alamar blue after treatment with n-dodecyl beta-D-maltoside (DDM) orTritonX-100 expressed relative to the metabolism of untreated H37Rv. The mean+/−standard deviations of three independent biologic replicates are expressed. Strains lacking ESX1 function, espACD and EspA disulfide bond formation were significantly more susceptible to DDM and TritonX-100 as compared to H37Rv as determined by T-test (for these comparisons, all p<0.005).